yourSIAL NGS Service

To better facilitate and meet the needs of our Clients, thanks to a team of experts, Sial S.r.l. offers next-generation sequencing (NGS) service in different applications such as transcriptome analysis, exome analysis, metagenomics and epigenomics, bioinformatics and microarray services for DNA methylation analysis and genotyping.

Our team works closely with you to provide a high-quality sequencing and bioinformatics analysis service, with the possibility of tailoring the service to your specific project needs, as well as to provide excellent results for scientific publication.

Learn about all the services available.

Products

01

yourSIAL RNA sequencing

Using the NGS technique applied to RNA, it is possible to analyze the transcriptome, or the set of RNA molecules in a cell, and determine when and where each gene is activated or deactivated in an organism's cells and tissues. In addition to the analysis of gene expression changes, RNAseq can be used to identify alternative splicing events, gene fusions and expression isoforms, to analyze unknown transcripts and for RNA editing, as well as for the analysis of noncoding RNAs.

Stranded mRNA sequencing

Stranded mRNA sequencing is a sensitive and accurate method for analyzing gene expression in a targeted set of genes of interest.
This type of service allows identification of known and novel transcriptome isoforms, alternative splicing events, fusion genes, and quantification of allele-specific expression.

Service specifications:
Stroke type: Paired end
Depth: 30 M reads/sample
No. sequenced base pairs: 2 x 75 (optional 2 x 150. Contact Sial Customer Care for more information)
Required starting sample qty: 300/500 ng to 1 µg diluted in a volume of about 20 µl (in case of low concentrated samples, a protocol for small amounts can be applied. For more information, please contact Sial Customer Care).
Allowable RINe value: 8 to 10.

Please note: Prior to submission, a quality check of the samples is possibly requested, which will also be performed by Sial laboratory technical staff, however.

    N.B. For samples larger than 5 download and fill in the excel file

    Applicant data

    Stranded total RNA sequencing

    Total RNA sequencing by NGS technique, allows us to obtain a complete image of the transcriptome, which is useful for analyzing both coding and noncoding RNA (ncRNA), especially long noncoding RNA (lncRNA). This type of service is useful for identifying fusion genes, known and novel transcripts, quantifying allele-specific expression, then polymorphisms present on RNA and analysis of noncoding RNAs.


    Service specifications:
    Stroke type: Paired end
    Depth: 30 M reads/sample
    No. sequenced base pairs: 2 x 75 (optional 2 x 150. Contact Sial Customer Care for more information).
    Species: bacteria, human, mouse, rat
    Sample type: FFPE, cells, blood
    Required starting sample qty: 300/500 ng to 1 µg diluted in a volume of about 20 µl (in case of low concentrated samples, a protocol for small amounts can be applied. Contact Sial Customer Care for more information).

    Please note: Prior to submission, we possibly require quality control of the samples, which will also be performed by Sial laboratory technical staff in any case

      N.B. For samples larger than 5 download and fill in the excel file

      Applicant data

      Small RNA sequencing

      Small RNA sequencing is a useful method to analyze small RNAs such as miRNAs, siRNAs and piRNAs in a single sequencing run, and thus study their biological role, as they are normally involved in gene silencing and post-transcriptional regulation of gene expression and how the same contributes to the phenotype.


      Service specifications:
      Stroke type: single-reads
      Depth: 20 M reads/sample
      No. base pairs sequenced: 1 x 75
      Species: Human, rat, mouse, plant, drosophila, nematode, zebrafish, any species
      Sample type: FFPE, cells, blood
      Required starting sample qty: from 100 ng (a protocol for small quantities can be applied. Contact Sial Customer Care for more information)

      Please note: Prior to submission, a quality check of the samples is possibly requested, which will also be performed by Sial laboratory technical staff, however.

        N.B. For samples larger than 5 download and fill in the excel file

        Applicant data

        02

        yourSIAL DNA sequencing

        The NGS technique applied to the DNA of any organism makes it possible to analyze by sequencing very large regions of the genome and gene pools. This tool offers several advantages such as:
        - Scalability: sequencing large regions of DNA in parallel, with productivity advantages over Sanger sequencing;
        - High resolution: it is possible to analyze base-by-base a gene, the exome or the entire genome;
        - Provides quantitative measurements based on signal strength;
        - Detects all types of genomic DNA alterations, including single nucleotide variations, insertions and deletions, copy number changes, and chromosomal aberrations;
        - High productivity and flexibility: multiple samples can be analyzed simultaneously.

        Exome Sequencing

        Exome sequencing has been recognized by the scientific community as a valid method for fast and accurate clinical diagnosis of various diseases.
        This type of method is also useful for researchers because it allows them to sequence multiple genes simultaneously and identify gene variants accurately. The yourSIAL Exome sequencing service identifies variants and in-depth analysis of coding mutations. The estimated number of exomes sequenced per run is calculated with an average coverage of 50X and a 2 × 150 bp read length. This method, was optimized to provide uniform and specific coverage of 45 Mb of exonic content (about 214,405 exons).

        Service specifications:
        Stroke type: paired-end
        No. of sequenced base pairs: 2 x 150 bp
        Default enrichment type: Illumina enrichment kit, about 45 Mb
        Coverage: 50x (optional 100x. Contact Sial Customer Care for more information)
        Required starting sample quantity: from 100 ng
        Source matrix: FFPE, circulating DNA, blood, saliva
        Species: Man

          * For samples larger than 5 download and fill out excel file

          Applicant data

          Genome Wide Screening

          yourSIAL Genome Wide Screening leverages array technology to offer a high-quality, reproducible solution for population-scale genetic studies, variant screening and precision medical research. The type of service offered is useful for association studies, typing samples and analyzing polymorphisms from different types of samples such as saliva, oral swabs, blood, fresh tissue, paraffin-embedded and formalin-fixed samples.

          Service specifications:
          No. probes: 654,000 markers
          Source matrix: FFPE, circulating DNA, blood, saliva, buccal swab
          Required starting sample quantity: from 300 ng
          Technology: Microrarray

            * For samples larger than 5 download and fill out excel file

            Applicant data

            03

            youSIAL Epigenomic sequencing

            Epigenomics is the study of DNA organization at the genomic level, which is useful for assessing gene regulation at the transcriptional level, to identify protein binding sites to DNA or hypo- and hyper-methylated regions within the genome. Using methylation assays, for example, it is possible to obtain quantitative data on the methylation status at the level of CpG sites and thus to be able to understand, with good resolution, epigenetic changes.

            Human Methylation EPIC

            Using yourSIAL Human MethylationEPIC service, it is possible to analyze by array technique, more than 850,000 methylation sites and thus analyze single nucleotide polymorphisms. Multiple samples, can be analyzed in parallel, even in FFPE, and from small quantities.

            Service specifications:
            No. of probes: 850,000
            Species: Man
            Source matrix: FFPE
            Required starting sample quantity: from 250 ng
            Technique: Microarray

              N.B. Per campioni maggiori di 5 scarica e compila il file excel

              Dati richiedente

              Metagenomic

              Sequencing of 16S RNA is the main application of amplicon sequencing in metagenomics, which makes it possible to determine evolutionary relationships among microorganisms, since it is a relatively large molecule, endowed with the same function in every organism, involved in protein synthesis and therefore aaracterized by highly conserved regions. At the same time, 16S gene sequences are characterized by highly variable regions that exhibit homology
              only among bacteria of the same species and can therefore be exploited for phylogenetic analysis.

              The type of service offered begins with DNA extraction (optional) from different types of samples such as feces, soil, water, filters, skin, vaginal swabs, and endometrium; followed by primer amplification for the V3 and V4 variable regions of the 16S rRNA gene and concluding with sequencing on Illumina platform.

              1st level bioinformatics analysis is included in the service, that is, a taxonomic identification report (order - family - genus - species) is provided.

              Service specifications:
              Amplicon type: 16 S (also optional for 18S amplicon. Contact Sial Customer Care for more information)
              Depth: 100,000 reads/sample
              Length: 2x300
              Sample type: bacterial DNA or matrices such as feces, soil, water, filters, skin, vaginal swabs and endometrium
              Required starting sample quantity: from 50/100 ng
              Default primer type: V3/V4

                * Per campioni maggiori di 5 scarica e compila il file excel

                Dati richiedente

                04

                yourSIAL Comprehensive Oncology Panel

                yourSIAL Comprehensive Oncology Panel is a service to obtain a comprehensive genomic profile of tumor samples. It is possible to use this panel to identify all relevant DNA and RNA variants implicated in various types of solid tumors and to evaluate key immunoncology biomarkers, such as: microsatellite instability (MSI) and tumor mutational burden (TMB), a valuable tool to identify patients who can respond better to immunotherapy.
                The service includes a bioinformatics analysis, which is the release of a report of all identified genetic variants, along with a TMB value.

                yourSIAL Comprehensive Oncology Panel

                Service specifications:
                Panel type: 500 known cancer genes
                Sequencing type: paired-end, 2x100
                Sample type: FFPE or liquid biopsy samples
                Coverage: 400x
                Required starting sample quantity: from 100 ng

                Bioinformatics analysis included: report of gene variants and their locations, plus
                TMB value for assessment of global mutation status.

                  N.B. For samples larger than 5 download and fill in the excel file

                  Applicant data

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