Extraction of nucleic acids

Nucleic acid extraction is the basis of every molecular biology experiment. The technique has been tried and tested for quite some time now, although over the years technologies have been developed in step with advances in scientific research to help the researcher improve the yield and quality of nucleic acids.
These features are essential for all subsequent steps of gene expression analysis.

Starting from different biological matrices, Sial offers a wide range of kits for both manual and automated extraction of nucleic acids, in this case also providing the necessary instrumentation.

Discover our product catalog.

Products

01

Manual extraction kit

Norgen Biotek Corp.

This section includes several kits for purifying nucleic acids by spin columns from different biological matrices.
CE-IVD-marked kits are also available.
SIAL customer service can be contacted for more information.

Genomic DNA

Genomic DNA Isolation Kits

- Rapid, purifies by spin columns from different matrices such as tissues, cells, body fluids, buffers and viruses
- High yield
- Excellent quality
- Purified DNA is ready for use in downstream applications

24700
Genomic DNA Isolation Kit
50 preps

24750
Genomic DNA Isolation Kit
100 preps

24770
Genomic DNA Isolation Kit
250 Preps

Cells and Tissue DNA Isolation Kit

- Rapid, purifies by spin column
- High yield
- Excellent quality

The purified DNA is ready for use in downstream applications such as PCR, qPCR, genotyping, and more.

53100
Cells and Tissue DNA Isolation Kit
50 preps

Fungi/Yeast Genomic DNA Isolation Kit

- Rapid, purification of genomic DNA by spin column from yeast, fungal spores or mycelia, bacteria, including Gram-positive
- Tubes containing marbles (included in the kit) for mechanical homogenization

The purified DNA is ready for use in downstream applications such as PCR, qPCR, RFLP and more.

27300
Fungi/Yeast Genomic DNA Isolation Kit
50 preps

Bacterial Genomic DNA Isolation Kit

- Isolates genomic DNA from both Gram-positive and Gram-negative bacteria
- Rapid, purifies by spin column
- 96-well format for high throughput

Purified DNA is of high yield and quality is used in downstream applications such as PCR, qPCR, sequencing and more.

17900
Bacterial Genomic DNA Isolation Kit
50 preps
17950
Bacterial Genomic DNA Isolation 96-well Kit
192 preps

Phage DNA Isolation Kit

- Isolates genomic DNA from different phages
- High yield of total DNA
- Quick and easy protocol thanks to spin-column
- Extraction does not require the use of phenol or cesium chloride

High DNA yield, about 3-15 µg DNA from 106-1010 pfu/ mL of phage

46800
Phage DNA Isolation Kit
50 preps

46850
Phage DNA Isolation Kit
100 preps

Direct DNA Extraction Kit (Bacteria)

- Quick
- Simple protocol
- Reproducibility

Can be used with high throughput platforms

61500
Direct DNA Extraction Kit (Bacteria)
50 preps

Plasmid DNA

Plasmid Miniprep Kit

- High yield of plasmid DNA up to 13 kb
- The obtained Plasmid DNA is ready for restriction enzyme digestion, transformation, sequencing, and more
- Also available in 96 well format as well as single spin column

13300
Plasmid MiniPrep Kit
50 preps

46400
Plasmid MiniPrep Kit
250 preps

25900
Plasmid MiniPrep Kit – 96 well
1 x 96 well

43300
Plasmid MiniPrep Kit – 96 well
4x 96 well

Plasmid DNA Maxiprep Kit

- High yield of plasmid DNA up to 13,000 bp from 100 ml of bacterial culture
- Fast and efficient protocol through spin column

The obtained Plasmid DNA is ready for restriction enzyme digestion, transformation, sequencing and more.

46500
Plasmid DNA Maxiprep Kit
4 preps

46600
Plasmid DNA Maxiprep Kit
20 preps

BAC DNA Miniprep Kit

- Quick and simple protocol by alcohol precipitation or column spin;
- High yield - up to 1 µg from 3 ml of culture;
- Versatile protocol adaptable to different starting volumes

18050
BAC DNA Miniprep Kit
50 preps

RNA

Total RNA Purification Kit

Several RNA extraction kits are available as kits for total RNA, including miRNAs, which do not require phenol steps but rely on extraction by column.
These kits bind and elute all RNA regardless of size and GC content, without the use of beads.
They are available in different formats and vary from each other in starting sample, final yield, etc.
A series of kits are also available for: RNA extraction from animal tissues stored in RNAlater® or Trizol®, for microRNA in 30', for adipose tissues, plants and fungi, and for RNA/DNA/Protein.
You can learn more by downloading the brochure of the various RNA kits available.

17200
Total RNA Purification Kit
preps

37500
Total RNA Purification Kit
100 preps

17250
Total RNA Purification Kit
250 preps

02

Extraction kit using magnetic marbles

Omega Biotek

This section includes several kits for nucleic acid purification using magnetic bead technology.

RNA

Mag-Bind® Total RNA 96 KiT

The kit allows purification of RNA from 5-10 mg of tissue or 1 x 106 cells in culture. Unlike column-based systems, with the MagBind® Total RNA 96 kit purification is done by paramagnetic beads resulting in increased kinetics and binding efficiency. This kit also is compatible with several automated stations such as:
Hamilton Microlab® STAR
Hamilton Microlab® NIMBUS
KingFisher™, BioSprint®, and MagMAX® 96

M6731-00
Mag-Bind® Total RNA 96 Kit
1 X 96 preps

M6731-01
Mag-Bind® Total RNA 96 Kit
4 X 96 preps

DNA

Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

The kit allows DNA purification from whole blood, buccal swabs, saliva, mouse tail sections and dried blood spot. Unlike column-based systems, the Mag-Bind® Blood & Tissue DNA HDQ kit takes advantage of paramagnetic bead technology to obtain high-quality DNA for use in downstream applications such as NGS, qPCR, PCR and microarrays.
For more information you can download the brochure or contact Sial customer care.

M6399-00
Mag-Bind® Blood & Tissue DNA HDQ 96 Kit
1 X 96 preps

M6399-01

Mag-Bind® Blood & Tissue DNA HDQ 96 Kit
4 X 96 preps

cfDNA

Mag-Bind® cfDNA Kit

The Kit allows rapid purification of circulating DNA from 500-10,000 µL of plasma or serum. It can be used manually through a magnetic plate or with automated systems.
The system combines the reversible binding properties of nucleic acids to paramagnetic Mag-Bind® beads with a unique binding system that captures smaller fragments of DNA (150-400 bp) thereby minimizing the binding of larger fragments, such as gDNA.
For more information you can download the brochure or contact Sial customer care.

M3298-00
Mag-Bind® cfDNA Kit
5 preps

M3298-01
Mag-Bind® cfDNA Kit
50 preps

M3298-02
Mag-Bind® cfDNA Kit
200 preps

DNA/RNA

Mag-Bind® TotalPure NGS

The Mag-Bind® TotalPure NGS Kit offers an easy solution for DNA and RNA purification for the NGS technique with high recovery rates. The purified DNA and RNA can be used for a variety of downstream applications such as library preparation for NGS, microarrays, automated fluorescent sequencing, and restriction enzyme digestion.

The kit is designed for both manual and automated use with the following tools:
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- KingFisher™, BioSprint®, and MagMAX® 96

For more information you can download the brochure or contact Sial customer care.

M1378-00
Mag-Bind® TotalPure NGS
5 ml

M3298-01
Mag-Bind® TotalPure NGS
50 ml

M3298-02
Mag-Bind® TotalPure NGS
500 ml

03

Tools and kits for automated extraction

BigFish, Bio Tech

This section includes machines and related kits for automated extraction by magnetic beads of nucleic acids from different samples.

Automated nucleic acid extraction system

BIG FISH™ Nuetraction 32/96 Nucleic Acid Purification System

The BIG FISH™ Nuetraction 32/96 Nucleic Acid Purification System is an automated system for extracting nucleic acids using magnetic beads from various samples such as whole blood, tissues, cells, bacteria, plasmids, viruses, plants and more.

The compact-sized instrument is equipped with a UV lamp for decontamination, a temperature control system and a touch screen. Therefore, it does not need an external pc as it is possible to select the protocol of interest directly on the instrument or to customize and save them for later use.

Two versions of the BIG FISH™ Nuetraction extraction system are currently available: 32 or 96 samples. Each extraction kit contains all the necessary materials such as reagents i.e., marbles, wash buffer I and II and eluition buffer, loaded into sealed deep wells that simply need to be peeled and loaded onto the instrument after adding the sample to be purified, and disposable plastic material. To avoid reagent waste when purifying only one sample, a kit was developed for the 32-sample extraction system where reagents are loaded into individual tubes and not into deep well plates.

The BIG FISH™ Nuetraction 32/96 Nucleic Acid Purification System is the ideal solution for your laboratory.

HBB-BFEX-32
BIG FISH™ Nuetraction 32 Nucleic Acid Purification System
32 samples

HBB-BFEX-96
BIG FISH™ Nuetraction 96 Nucleic Acid Purification System
96 samples

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