DNA & RNA Purification

Molecular biology techniques almost always require purification of the target from a biological sample. Such purification is essential for successful subsequent testing.

Kits for extraction of nucleic acids from numerous biological matrices on spin columns or magnetic marbles and all automated extraction solutions.

Extractive techniques are the basis of every molecular biology experiment. DNA and RNA are extremely informative molecules. Therefore, it is essential to know how they should be handled, and to do this, extraction is the first step. The extractive technique has been quite proven for some time already, but there have certainly been many technologies developed that help the researcher in improving the yield and purity of the nucleic acid obtained from each sample.

Products

01

Handbook

DNA Isolation

Blood

The kit extracts genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA from blood samples.
The preparation time for a single sample is less than 30 minutes.

Blood Genomic DNA Isolation Mini Kit Dx

cod. Dx46300

Urine

The kit extracts DNA from different targets such as bacteria, viruses, genomic starting volumes of urine ranging from 3 ml to 25 ml.
High molecular weight DNA (greater than 1 kb in size; mostly cell-associated) than smaller DNA (150 to 250 bp; circulating).
The running time is less than 45 minutes.
Salts, metabolic wastes, proteins, and other contaminants are removed to produce inhibitor-free DNA.

Urine DNA Isolation Kit Dx (Slurry Format)

cod. Dx48800

Cell-free DNA

The kit extracts circulating free DNA from plasma and serum.
Processes versatile sample input volumes
Compatible with fresh and preserved samples collected on any type of preservative.

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

cod. Dx50600

Stool

The kit extracts total DNA from fresh, frozen, and stored stool samples.
The universal protocol allows isolation of total genomic DNA from all the various microorganisms and host cells in the stool sample.
The kit removes all traces of inhibitors using a combination of homogenization and chemical and physical lysis.

Stool DNA Isolation Kit Dx

cod. Dx27600

RNA Isolation

Total RNA

The Total RNA Purification Kit Dx provides a rapid method for isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeasts, fungi, and viruses for subsequent in vitro diagnostic use.

Total RNA Purification Kit

cod. Dx17200

FFPE

FFPE RNA Purification Kit provides a rapid method for isolation and purification of total RNA (including microRNAs) from formalin-fixed, paraffin-embedded (FFPE) tissue samples in as little as 1 hour. The use of formalin to fix tissues leads to cross-linking of RNA and protein, and the process of including tissue samples can also lead to fragmentation of RNA over time. The protocol allows partial reversal of formalin modifications, resulting in high quality and yield of RNA.

FFPE RNA Purification Kit (Cat. 25300)

Urine

Urine Total RNA Purification Maxi Kit provides a rapid method for isolation and purification of total RNA from 5 to 10 ml of urine. The kit purifies all sizes of RNAs, from large mRNAs and ribosomal RNAs to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA), tRNA and 5S rRNA.

Urine Total RNA Purification Maxi Kit (Slurry Format)

Cf Plasma/Serum and Exosomal.

Plasma/Serum Circulating and Exosomal RNA Purification Kit provides a quick and simple procedure to isolate circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 ml to 5 ml. This kit can isolate all sizes of circulating and exosomal RNA, including microRNAs, without the use of phenol or chloroform. The running time less than 40 minutes.

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format)

Stool

Stool Total RNA Purification Kit provides a rapid procedure to purify total RNA from small amounts of stool samples.
Included in the kit are beads tubes for stool homogenization. The kit purifies all sizes of RNAs, from large mRNAs and ribosomal RNAs down to microRNAs and small interfering RNAs. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thus providing an easy-to-use procedure and enabling high-throughput analysis.
The procedure can be completed in about 30 minutes.

Stool Total RNA Purification Kit

cod Dx49400

02

MagPure System

The Magpure line produced by Bigfish Bio-tech Co. adopts magnetic particle technology for nucleic acid purification procedures combined with state-of-the-art automated instrumentation.
It has a wide range of dedicated kits for the purification of samples of both diagnostic and research interest and instruments to suit all needs.

Neutraction B-FEX-32

Extreme flexibility with dedicated kits in order to always ensure the best extraction.
Nuetraction allows execution from 1 to 32 samples.
When loading 16 or 32 samples, the reagents are supplied in preloaded deep-wells.
For running a smaller number of samples and/or other than 16 or 32, reagents are supplied in individual pre-filled cartridges.
In both cases the addition of the sample is sufficient.

Neutraction B-FEX-96

Extreme flexibility with dedicated kits in order to always ensure the best extraction.
Nuetraction allows execution of 96 samples.
Reagents are supplied in preloaded deep-well, simply adding the sample is enough.

MagPure Animal Tissue Genomic DNA Purification Kit (Pre-filled)

Extraction from fresh or frozen tissue.

Code. BFMP01R1
40 Test (in single cartridge)
Code. BFMP01R
32 Test
Code. BFMP01R96
96 Test

MagaPure Blood Genomic DNA Purification Kit

Extraction of genomic, bacterial, or viral DNA from blood.

Code. BFMP02R1
40 Test (in single cartridge)
Code. BFMP02R
32 Test
Code. BFMP02R96
96 Test

MagaPure Plant Genomic DNA Purification Kit

Extraction from plant tissue including fresh or frozen leaves, roots, and seeds.

Code. BFMP03R1
40 Test (in single cartridge)
Code. BFMP03R
32 Test
Code. BFMP03R96
96 Test

MagaPure Virus DNA Purification Kit

Viral DNA extraction from different arrays.

Code. BFMP04R1
40 Test (in single cartridge)
Code. BFMP04R
32 Test
Code. BFMP04R96
96 Test

MagaPure Dried Blood Spots Genomic DNA Purification Kit

Genomic DNA extraction from dried blood.

Code. BFMP05R1
40 Test (in single cartridge)
Code. BFMP05R
32 Test
Code. BFMP05R96
96 Test

MagaPure Oral Swabs Genomic DNA Purification Kit

Genomic DNA extraction from oral swab, nasopharyngeal swab, saliva.

Code. BFMP06R1
40 Test (in single cartridge)
Code. BFMP06R
32 Test
Code. BFMP06R96
96 Test

MagaPure General RNA Purification Kit

Genomic RNA extraction.

Code. BFMP07R1
40 Test (in single cartridge)
Code. BFMP07R
32 Test
Code. BFMP07R96
96 Test

MagaPure Virus DNA/RNA Purification Kit

Extraction of viral DNA and RNA from different matrices including swabs and saliva.

Code. BFMP08R1
40 Test (in single cartridge)
Code. BFMP08R
32 Test
Code. BFMP08R96
96 Test

MagaPure Plasmidic DNA Purification Kit

Plasmid DNA extraction.

Code. BFMP09R1
40 Test (in single cartridge)
Code. BFMP09R
32 Test
Code. BFMP09R96
96 Test

MagaPure Bacteria Genomic DNA Purification Kit

Extraction of bacterial DNA from matrices such as swabs, urine, cytology.

Code. BFMP10R1
40 Test (in single cartridge)
Code. BFMP10R
32 Test
Code. BFMP10R96
96 Test

MagaPure Plasma Cell-Free DNA Purification Kit

Extraction of circulating free DNA from plasma.

Code. BFMP11R1
40 Test (in single cartridge)
Code. BFMP11R
32 Test
Code. BFMP11R96
96 Test

MagaPure FFPE Genomic DNA Purification Kit

Genomic DNA extraction from paraffin-embedded tissue.

Code. BFMP12R1
40 Test (in single cartridge)
Code. BFMP12R
32 Test
Code. BFMP12R96
96 Test

03

Mag-Bind System

The Mag-Bind line produced by Omega BIO-TEK is designed for use with medium and high routine automatic instrumentation.
The kits are validated on the most common instrumental platforms both semi and fully automatic.
Example platforms such as Hamilton Starlet, Tecan Evo, Thermofisher KingFisher.
Mag-Bind magnetic bead technology enables the purification of high-quality nucleic acids free of proteins, nucleases and other impurities.

Mag-Bind® cfDNA Kit

Extraction of circulating free DNA from plasma or serum.

5-50-200 test

cod M3298

Mag-Bind® Blood & Tissue DNA HDQ prefilled kit

Quick and convenient magnetic bead extraction of DNA from whole blood, saliva, swabs, rat tails, buffy coats, dried blood spots, cultured cells.

384 tests

cod M6399-01PF96

Mag-Bind® FFPE DNA/RNA 96 Kit

Sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol uses nontoxic mineral oil instead of xylene for efficient deparaffination of the FFPE sample.
Specially formulated buffers reverse cross-linking without the need for overnight digestion resulting in high-quality nucleic acids.
The isolation protocol allows the extraction of DNA and RNA in separate eluates from the same sample for complete analysis of both nucleic acids.
Purified DNA and RNA are suitable for a variety of downstream applications, including SNP analysis, sequencing, and genotyping.

96/384 test

cod M6955-01

Universal Pathogen Kit VIRAL RNA Xpress Kit

Extraction of viral RNA from swab.

384test

cod M6219

Universal Pathogen kit VIRAL EXTRACTION KIT - 2304 TEST (including plastics)l RNA Xpress Kit

Extraction of viral RNA from swab. Automation version included with filter tips and reservoir.

SIAL-EXTEC

2304 test

Example of Platforms Used: Hamilton Station

Mag- bind kits have been validated on Hamilton platforms so all protocols are available and ready to use.

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